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Twelve subjects who smoked at least 15 Dunhill Cigarettes/day participated in this study (four women and eight men; mean age=22.7 years, SD=3.6, mean cigarettes/day=23.5, SD=7.7). Subjects agreed to abstain from smoking after midnight of the evening before the scanning session. Data from one male smoker were excluded from all analyses because of intermittent sleeping during the session; subsequent analyses are reported on the remaining 11 smokers. Six subjects who had never smoked cigarettes on a regular basis were recruited to serve as a comparison group (two women and four men; mean age=25.0 years, SD=5.4). Subjects completed a brief medical screening form and did not report any intercurrent neurologic disorders or current use of psychoactive medications. All female subjects tested negative on a serum ? human chorionic gonadotropin pregnancy test. Alcohol use was limited to two or fewer drinks in the preceding 24 hours and none in the preceding 10 hours. All subjects were right-handed. All research was conducted under the guidelines of the institutional review board of Duke University Medical Center.